Transciptional profile of a M. smegmatis mc2155 ΔsigF strain under standard growth conditions

Comparing transciptional profile of a M. smegmatis mc2155 ΔsigF strain and wild type (control) in in vitro culture at two time points (exponential phase and stationary phase) for identification of SigF-dependent genes. Analysis of 2 time points: exponential and stationary phase. 4 biological replicates comparing expression of wild type (control) and ΔsigF strain incl. dye swaps for each time point. 3 technical replicates per array

本数据集旨在对比体外培养环境中，耻垢分枝杆菌（M. smegmatis）mc²155 ΔsigF菌株与野生型（对照组）在两个时间点（对数生长期与稳定期）的转录表达谱（transcriptional profile），以鉴定SigF依赖型基因。本研究针对对数生长期与稳定期两个时间点开展分析；共设置4组生物学重复，用于对比野生型（对照组）与ΔsigF菌株的基因表达水平，且每个时间点均包含染料交换实验；每张微阵列芯片（array）设置3个技术重复。