Data to support Conservation genetics of a freshwater percid, <i>Sander canadensis</i>, in Manitoba, Canada

We used twelve microsatellite loci to resolve genetic diversity and structure of sauger (<i>n</i>= 872) across nine waterbodies in the Nelson-Churchill watershed. Five waterbodies contained low numbers of walleye × sauger hybrids. Overall, genetic diversity were highest in Lake Winnipeg, the largest lake in the watershed, and lowest in Lakes Manitoba and Winnipegosis. Despite their close geographic proximity, genetic differentiation between Lake Winnipeg and Lake Manitoba/Lake Winnipegosis were moderately high and were found to have low levels of historical and on-going gene flow. On the other hand, high connectivity, based on low levels of genetic differentiation and high estimates of on-going gene flow were found between Lake Winnipeg and the Red River, Assiniboine River, Lac du Bonnet, and Stephens Lake, which span a larger geographic area but are more connected in the watershed. Clustering and genetic differentiation-based methods indicated that fish in Lake Winnipeg and Lake Manitoba/Lake Winnipegosis belong to different genetic groups, but most individuals from all sites exhibited some level of ancestral admixture, particularly fish sampled in the river systems.本研究利用12个微卫星位点（microsatellite loci），对纳尔逊-丘吉尔流域（Nelson-Churchill watershed）内9个水体中的872尾加拿大梭鲈（sauger）开展遗传多样性与群体遗传结构解析。其中5个水体中存在少量大眼梭鲈（walleye）与加拿大梭鲈的杂交个体。总体而言，作为该流域最大湖泊的温尼伯湖（Lake Winnipeg）遗传多样性最高，而马尼托巴湖（Lake Manitoba）与温尼伯戈西斯湖（Lake Winnipegosis）的遗传多样性最低。尽管温尼伯湖与马尼托巴湖、温尼伯戈西斯湖地理毗邻，但三者间的遗传分化程度处于中等偏高水平，且历史与当前的基因流（gene flow）水平均较低。与之相反，温尼伯湖与雷德河（Red River）、阿西尼博因河（Assiniboine River）、博内特湖（Lac du Bonnet）及斯蒂芬斯湖（Stephens Lake）之间虽地理跨度更大，但流域内种群连通性更强——这一结论基于二者间较低的遗传分化水平与较高的当前基因流估算值。聚类分析与基于遗传分化的分析方法均显示，温尼伯湖与马尼托巴湖、温尼伯戈西斯湖中的鱼类属于不同的遗传类群，但所有采样点的多数个体均存在一定程度的祖先血统混合，其中尤以河流水系中的采样个体最为显著。