Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma [expression]

Transcriptional profiling of two pediatric human DIPG cell lines, harboring the K27M mutation. DIPG cell lines were treated with GSKJ4 for 24 and 72 h. The untreated cell lines served as baseline, and expression fold changes in each treated cell line were determined relative to the baseline. Goal was to determine the effects of GSKJ4 treatment on global gene expression in DIPG cells harboring the K27M mutation. Three-condition experiment: 72 h GSKJ4 treated cells vs. 24 h GSKJ4 treated cells vs. untreated cells. Biological replicates: 1 cell line, with 2 technical replicates per condition.

本数据集针对两株携带K27M突变的儿童源性人弥漫内生型桥脑胶质瘤（DIPG）细胞系开展转录组谱分析。将该两株细胞系分别用GSKJ4处理24小时与72小时，以未处理的细胞系作为对照基线，计算各处理组细胞系相对于该基线的基因表达倍数变化。本研究旨在明确GSKJ4处理对携带K27M突变的DIPG细胞全局基因表达的影响。本实验包含三组对照：72小时GSKJ4处理组、24小时GSKJ4处理组与未处理组。生物学重复设置为仅使用1株细胞系，每个实验条件设置2次技术重复。