VIB1, a link between glucose signaling and carbon catabolite repression, is essential for plant cell wall degradation by Neurospora crassa. Neurospora crassa

Purpose: To explore the function of VIB1 in regulating cellulase production in Neurospora crassa. Method: mRNA from vib-1 mutants were collected after a culture shift from sucrose to Avicel (crystaline cellulose) or carbon-free media. Expression profiles of vib-1 mutants were compared with published profiles of wild type created under the same conditions. Results: We found that many genes that specifically upregulated in wild type upon exposure to Avicel were expressed at low levels in ∆vib-1 and many other genes involved in metabolism and energy were expressed at high levels compared to wild type. Conclusions: Our study shows that VIB1 is required for suppression of glucose response and carbon catabolite repression to allow proper expression of clr-2 and subsequent cellulase production in response to cellulosic induction. Overall design: Biological triplicates of liquid culture N. crassa were harvested at 4 hours after a switch from sucrose media to media of interest. Global mRNA abundances were measured by sequencing on the Illumina Genome Analyzer Iix and HiSeq2000 platforms.

研究目的：探讨VIB1在粗糙脉孢菌（Neurospora crassa）纤维素酶生产调控中的功能。
实验方法：将菌株从蔗糖培养基转接至微晶纤维素（Avicel，结晶纤维素）或无碳源培养基培养后，收集vib-1突变体的信使RNA（mRNA）。将vib-1突变体的表达谱与相同条件下已发表的野生型表达谱进行比对分析。
研究结果：本研究发现，野生型菌株暴露于Avicel后特异性上调的大量基因在Δvib-1突变体中呈低表达状态；而诸多参与代谢与能量代谢过程的基因在突变体中的表达水平显著高于野生型。
研究结论：本研究表明，VIB1对于抑制葡萄糖响应及碳分解代谢物阻遏是必需的，以确保clr-2的正确表达及后续纤维素酶在纤维素诱导下的产生。
整体实验设计：将液体培养的粗糙脉孢菌（Neurospora crassa）从蔗糖培养基切换至目标培养基后4小时，收获3份生物学重复样本。采用Illumina Genome Analyzer Iix及HiSeq2000测序平台对全局mRNA丰度进行测序检测。