LncRNA EPR transcriptional activity controls intestinal mucus and prevents susceptibility to inflammation and tumorigenesis [RNA-seq]

The lncRNA EPR (a.k.a BC030870) is highly enriched in the gastrointestinal tract in mice and, more specifically, in the large intestine. We generated conditional EPR knock-out in large intestine e and analyzed genome-wide the gene expression changes by RNA-Seq. Total RNA was extracted from the proximal colon of six control (EPR fl/fl) and six knock-out (EPR cKO) mice. Libraries were constructed and sequenced. Results indicate that EPR knock-out predominantly leads to down-regulation of genes implicated in mucus biogenesis. These results help in explaining the phenotype displayed by EPR cKO mice that is characterized by higher susceptibility to intestinal inflammation and cancer formation. Overall design: Total RNA was extracted from the proximal colon of six control (EPR fl/fl) and six knock-out (EPR cKO) mice.

长链非编码RNA（long non-coding RNA，lncRNA）EPR（别名BC030870）在小鼠胃肠道中高度富集，且在大肠内富集程度尤为显著。本研究构建了大肠组织中的EPR条件性敲除模型，并通过RNA-Seq对全基因组范围内的基因表达变化进行了分析。我们从6只对照组（EPR fl/fl）小鼠与6只EPR条件性敲除（EPR cKO）小鼠的近端结肠中提取总RNA，随后构建测序文库并完成测序。结果表明，EPR敲除主要导致参与黏液生物合成的基因表达下调。该结果有助于解释EPR cKO小鼠所表现出的表型——这类小鼠对肠道炎症及肿瘤形成具有更高的易感性。实验整体设计：从6只对照组（EPR fl/fl）小鼠与6只EPR敲除（EPR cKO）小鼠的近端结肠中提取总RNA。