Proteomic analysis of small extracellular vesicles derived from human iPSC

Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinical translational potential in multiple aging-related degenerative diseases. Characterizing the PSC-sEVs is crucial for their clinical applications. However, the specific biomarker pattern of PSC-sEVs remains unknown. We displayed proteome analysis and further verification for identification of PSC-sEVs' specific biomarkers. The application of these specific biomarkers for PSC-sEVs identification may advance the clinical translation of PSCs-sEVs.

多能干细胞来源的小型细胞外囊泡（pluripotent stem cell-derived small extracellular vesicles，PSC-sEVs）已在多种衰老相关性退行性疾病中展现出显著的临床转化潜力。对PSC-sEVs进行表征对于其临床应用至关重要，然而其特异性生物标志物谱仍未明确。本研究开展蛋白质组学分析并辅以后续验证实验，以鉴定PSC-sEVs的特异性生物标志物。将此类特异性生物标志物应用于PSC-sEVs的鉴定，有望推动PSC-sEVs的临床转化进程。