Identification of differential expressed genes after ELK4 knockdown in colorectal cancer cells. Identification of differential expressed genes after ELK4 knockdown in colorectal cancer cells

We have demonstrated that ELK4 promted progression and tumorigenesis in colorectal cancer. In order to elucidate the transcriptional regulation mechanism of ELK4 in colorectal cancer, RNA-seq analysis was performed on ELK4 knockdown and control HCT116 cells. Overall design: HCT116 cells with ELK4 stable knockdown were generated by three independent shRNAs.

本研究已证实ELK4（ELK4）可促进结直肠癌的进展与肿瘤发生。为阐明ELK4在结直肠癌中的转录调控机制，本研究对ELK4敲低组与对照组HCT116细胞开展了RNA测序（RNA-seq）分析。实验整体设计：通过3条独立的短发卡RNA（shRNAs）构建得到ELK4稳定敲低的HCT116细胞。