Levels of phenylpropanoids and iridoids in extracts and infusions of Verbena minutiflora

Abstract Herein we report for the first time the levels of phenylpropanoids and iridoids in extracts and infusions of V. minutiflora consumed in Brazil to treat urinary and infectious disorders. An in house validation study demonstrated good accuracy and precision to determine the bioactive compounds in V. minutiflora by HPLC-DAD. Phenylpropanoids varied in the extracts (leaves 139.70 to 221.20 mg g-1, flowers 106.43 to 227.22 mg g-1, stems 42.18 to 56.48 mg g-1). Verbascoside occurred in higher concentration in extracts of leaves (87.66 – 136.16) mg g-1 and flowers (58.12 – 148.96) mg g-1 than in stems (19.24 – 24.62) mg g-1. Iridoids in extracts were as follows: leaves (46.60 − 54.79) mg g-1, flowers (55.88 − 93.87) mg g-1 and stems (40.05 to 61.74) mg g-1. High levels of iridoids (314.70 − 415.10) μg mL-1, phenylpropanoids (1996.39 − 2674.13) μg mL-1 and verbascoside (1029.38 − 1456.42 μg mL-1) in infusions support the popular consume of V. minutiflora.

摘要：本研究首次报道了巴西民间用于治疗泌尿系统及感染性疾病的V. minutiflora提取物与浸剂中苯丙素类（phenylpropanoids）和环烯醚萜类（iridoids）的含量水平。本实验室自主开展的方法学验证实验证实，采用高效液相色谱-二极管阵列检测器（HPLC-DAD）测定V. minutiflora中的生物活性化合物时，方法具备良好的准确度与精密度。不同部位提取物中的苯丙素类含量存在显著差异：叶片提取物为139.70~221.20 mg·g⁻¹，花提取物为106.43~227.22 mg·g⁻¹，茎提取物为42.18~56.48 mg·g⁻¹。毛蕊花苷（verbascoside）在叶片提取物（87.66~136.16 mg·g⁻¹）与花提取物（58.12~148.96 mg·g⁻¹）中的含量显著高于茎提取物（19.24~24.62 mg·g⁻¹）。各部位提取物中的环烯醚萜类含量分别为：叶片46.60~54.79 mg·g⁻¹，花55.88~93.87 mg·g⁻¹，茎40.05~61.74 mg·g⁻¹。浸剂中检测到的环烯醚萜类（314.70~415.10 μg·mL⁻¹）、苯丙素类（1996.39~2674.13 μg·mL⁻¹）以及毛蕊花苷（1029.38~1456.42 μg·mL⁻¹）含量较高，这为V. minutiflora的民间药用应用提供了科学依据。