Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.

We have grown C6 glioma cells and rat astrocytes, as well as astrocyte cells co-cultured together with C6 glioma. We performed proteome-wide LC-MS analysis of this experimental groups. The data including LC-MS/MS raw files and exported MaxQuant report. For our co-cultivated in vitro model we used astrocytes and C6 glioma cells. Astrocytes cell lines isolated from rat brain tissue. We analyzed astrocytes in two conditions: beforeand after co-cultivation. Proteins were assessed in an untargeted label-free bottom-up proteomic experiment using IDA approach (i.e. InformationDependent Acquisition) on AB Sciex TripleTOF 6600 Q-TOF mass-spectrometer coupled with LFQ (label-free quantification) approach by MaxQuant software. Dataset covers 165 samples (11 biological rand 5 technical replicates)

本研究培养了C6胶质瘤细胞、大鼠星形胶质细胞，以及与C6胶质瘤细胞共培养的星形胶质细胞。针对该实验组，我们开展了全蛋白质组液相色谱-质谱（Liquid Chromatography-Mass Spectrometry, LC-MS）分析。数据集包含液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）原始文件及导出的MaxQuant分析报告。本研究的体外共培养模型采用星形胶质细胞与C6胶质瘤细胞构建，其中星形胶质细胞系分离自大鼠脑组织。我们对两种状态下的星形胶质细胞进行了分析：共培养前与共培养后。本研究采用非靶向无标记自下而上蛋白质组学实验策略，通过AB Sciex TripleTOF 6600 Q-TOF质谱仪结合信息依赖性采集（Information-Dependent Acquisition, IDA）模式，并依托MaxQuant软件的无标记定量（Label-Free Quantification, LFQ）算法对蛋白质进行评估。本数据集共包含165份样本，其中包含11次生物学重复与5次技术重复。