Additional file 1 of Identification of kynurenine and quinolinic acid as promising serum biomarkers for drug-induced interstitial lung diseases

Additional file 1: Table S1. Raw data on biomarker concentrations and baseline characteristics of each DILD and DILD recovery sample. # Some samples could not be masured due to insufficient serum available for the quantitative analysis. Table S2. Raw data on biomarker concentrations and baseline characteristics of each serum sample other than DILD. Table S3. Metabolite peak areas measured by HILIC/TOF–MS in DILD serum samples. Table S4. PCR primer pairs used in this study. Table S5. Comparison of serum concentrations of conventional biomarkers among patient groups. Statistical differences between the two groups were examined using the Mann–Whitney U-test with Bonferroni correction. The adjusted p-values are presented in the table. The patients with missing values of the conventional biomarkers were excluded from the statistical analyses. DILD recovery: patients recovered from DILD, DILD-tolerant: the patient group taking similar medications to the DILD group but without DILD onset, BP: bacterial pneumonia, NTM: nontuberculous mycobacteriosis, IIPs: idiopathic interstitial pneumonias, CTD: lung disease associated with connective tissue disease, COPD: chronic obstructive pulmonary disease, BA: bronchial asthma, N.S.: not significant. Table S6. Positive rate of the identified DILD biomarker candidates and conventional biomarkers. The optimal cutoff values of KYN, QUNA, and KYN/TRP were determined using Youden’s index of the ROC curve built between the all-DILD and DILD recovery groups. The positivity rates for other conventional biomarkers were calculated using clinically used cutoff values. The cutoff values for biomarkers are indicated in the parenthesis. Table S7. DILD biomarker potential of tryptophan metabolites compared to serum biomarkers in OP and NSIP patients. AUROC values were calculated from ROC curves, and those with statistical significance (p-value < 0.05) are indicated in bold letters. DILD recovery: patients recovered from DILD, DILD-tolerant: the patient group taking similar medications to the DILD group but without DILD onset, BP: bacterial pneumonia, NTM: nontuberculous mycobacteriosis, IIPs: idiopathic interstitial pneumonias, CTD: lung disease associated with connective tissue disease, COPD: chronic obstructive pulmonary disease, BA: bronchial asthma, HC: healthy control, N.A.: not applicable.