Protein-SIP experiment with a bacterial community enriched from a sulfidic and benzene-contaminated aquifer

A benzene-degrading, sulfate-reducing enrichment culture originating from a benzene-contaminated aquifer was investigated by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). The time-resolved, quantitative analysis of carbon fluxes within the community supplied with either 13C-labeled benzene or 13C-labeled carbonate yielded different functional groups of organisms, with their peptides showing specific time dependencies of 13C relative isotope abundance indicating different carbon utilization.

本研究针对一株源自苯污染含水层的苯降解型硫酸盐还原富集培养物，采用基于蛋白质的稳定同位素探针（protein-SIP）结合基于宏基因组的功能宏蛋白质组学方法开展研究。对添加¹³C标记苯或¹³C标记碳酸盐的群落进行碳通量时间分辨定量分析后，鉴定得到不同功能类群的微生物；其肽段呈现出¹³C相对同位素丰度的特异性时间依赖性，表明这些类群存在不同的碳利用策略。