Expression data from LMP1-B cells. Expression data from LMP1-B cells

In this study, by microarray we analyzed gene expression changes due to LMP1 signaling in B cells, in order to identify candidate co-stimulatory ligands that may account for the ability of LMP1-B cells to induce cytotoxic CD4+ T cell response. The identified candidate co-stimulatory ligands were further validated by other functional assays. Overall design: In this experiment, two LMP1-expressing B cell samples were analyzed in comparison to two YFP-expressing control B cell samples.

本研究采用基因芯片（microarray）技术，分析B细胞中LMP1信号通路介导的基因表达变化，旨在筛选可解释LMP1转导B细胞诱导细胞毒性CD4+ T细胞应答能力的共刺激配体候选物。后续将通过其他功能实验对所鉴定出的候选共刺激配体进行验证。
实验设计：本实验设置2份表达LMP1的B细胞样本与2份表达YFP（yellow fluorescent protein）的对照B细胞样本，对两组样本开展对比分析。