Pseudomonas sp. CES Genome sequencing and assembly

The CES strain was isolated from soil at the University of Iowa Center for Biocatalysis and Bioprocessing using an enrichment procedure with caffeine as sole carbon and nitrogen source. Prior to isolation, coffee grounds had been amended to the soil for ten days. The soil was then collected and incubated in M9 minimum medium with .25 percent caffeine as described previously previously (Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.) The strain was initially characterized as Pseudomonas sp. Strain CES (for Coffee Enriched Soil) based on 16S rRNA sequencing and FAME analysis. CES uses the N-demethylation pathway for caffeine breakdown.

CES菌株系由爱荷华大学生物催化与生物转化中心的土壤中分离得到，该分离过程采用了一种以咖啡因作为唯一碳源和氮源的富集法。在分离之前，土壤中已混入了咖啡渣，持续十日。随后，土壤被收集并在M9最小培养基中进行培养，培养基中含0.25%的咖啡因，具体操作方法如前所述（Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.）。该菌株最初根据16S rRNA测序和脂肪酸甲酯（FAME）分析被鉴定为假单胞菌属的CES菌株（Coffee Enriched Soil）。CES菌株利用N-去甲基化途径进行咖啡因的分解。