Illumina RAD-seq reads for 190 dwarf birch (Betula nana) individuals in 36 populations in Scotland and Finland

We extracted DNA from 190 dwarf birch (Betula nana) individuals from 36 populations in Scotland and Finland. We used the restriction enzyme PstI. Cut DNA was normalized and submitted to FLORAGENEX (Portland, Oregon) for generation and sequencing of RAD tags following the protocol of Baird et al. (2008) and Hohenlohe et al. (2010). In brief, libraries were prepared with 96 unique 8-base barcodes, ligated via adaptors to PstI digested genomic DNA. The resulting fragments were sequenced in two lanes of an Illumina GAIIx platform with single-end 1x100bp chemistry. A single internal control sample generated from Saccharomyces bayanus was included in each lane. To match each set of reads with an individual and location, look at the "Sample title" column. The first two letters of each “Sample Title” correspond to population names and locations listed in Table S2 of Borrell et al 2018 Heredity

本研究从苏格兰与芬兰的36个种群中，采集190株矮桦（Betula nana）个体并提取其基因组DNA。实验采用限制性内切酶PstI对基因组DNA进行酶切，酶切产物经标准化处理后，送至位于美国俄勒冈州波特兰的FLORAGENEX公司，按照Baird等（2008）与Hohenlohe等（2010）的实验方案，完成RAD标签（RAD tags）的构建与测序。简言之，本次测序文库以96种独特的8碱基条形码（barcode）进行标记，通过接头将其与经PstI酶切的基因组DNA连接；所得酶切片段通过Illumina GAIIx测序平台的两个测序通道，采用单端1×100bp测序化学法完成测序。每个测序通道均设置了1株由巴氏酵母菌（Saccharomyces bayanus）制备的内部对照样本。若需将每组测序读段（reads）与对应个体及采样地点进行匹配，请查看"样本标题（Sample Title）"列。每个"样本标题（Sample Title）"的前两个字母，对应Borrell等2018年发表于《Heredity》期刊的补充表S2中所列的种群名称与采样地点。