Supplementary information and datasets: Transplant experiments on the establishment of stream bacteria in downstream lakes

This dataset contains raw and processed data generated as part of in vitro transplant experiments conducted using water collected from the catchment area of lake Siggeforasjön, central Sweden. Amplicon sequencing (16S rRNA genes and transcripts): Operational taxonomic unit (OTU) table (otu_table.txt). Associated taxonomy table containing assignments for all OTUs (taxonomy_table.txt). OTU sequences and bootstrap support values generated using the DADA2 taxonomic assignment are provided for each taxonomic level. Raw reads are available through the European Nucleotide Archive (ENA) at EMBL-EBI under accession number PRJEB108836. Flow cytometry: Flow cytometry measurements were used to quantify bacterial abundance and the relative proportions of high nucleic acid (HNA) to low nucleic acid (LNA) bacterial particles, expressed as the HNA/LNA ratio. Raw flow cytometry standard (.FCS) files from all analyzed samples are provided (FCS_files.zip). Bacterial protein production: Raw and processed bacterial protein production measurements based on leucine incorporation (Protein_production_raw_data.xlsx). The workbook includes: (i) raw measurements for samples, positive and negative controls; (ii) calculation steps used to derive bacterial protein production rates; and (iii) descriptions of all variables included in the calculations. Metadata table: Metadata file linking sample identifiers across datasets (Metadata.csv). Includes correspondence between sample name, sequencing read filenames (.fastq) deposited in ENA and associated flow cytometry (.FCS) files. Column descriptions are provided in Metadata_README.txt. Water flow: Water flow data (in m³ s⁻¹), originally obtained from the S-HYPE hydrological model through the Vattenwebb service of the Swedish Meteorological and Hydrological Institute. Daily data correspond to subcatchment SUBID 8110 (Siggeforasjön) for 2022. Files S1-S3: These supplementary files present relative abundance patterns of stream and lake communities across DNA- and RNA-based datasets (bacterial phylum or OTU level).本数据集包含瑞典中部锡格福拉松湖（Lake Siggeforasjön）集水区采集的水体用于体外移植实验所产生的原始与处理后数据。 扩增子测序（16S rRNA基因与转录本）： 包含操作分类单元（Operational Taxonomic Unit, OTU）表（otu_table.txt）。配套提供包含所有OTU分类注释信息的分类学表（taxonomy_table.txt），其中包含使用DADA2进行分类注释时生成的各分类层级的OTU序列与自举支持值。 原始测序读段可通过欧洲分子生物学实验室-欧洲生物信息学研究所（EMBL-EBI）旗下的欧洲核苷酸档案库（European Nucleotide Archive, ENA）获取，登录号为PRJEB108836。 流式细胞术： 本研究通过流式细胞术测量量化了细菌丰度，以及高核酸（High Nucleic Acid, HNA）与低核酸（Low Nucleic Acid, LNA）细菌颗粒的相对比例，并以HNA/LNA比值表示。所有分析样本的原始流式细胞术标准（.FCS）文件已打包为FCS_files.zip提供。 细菌蛋白生产： 包含基于亮氨酸掺入法的细菌蛋白生产测量原始与处理后数据（Protein_production_raw_data.xlsx）。该工作簿包含三部分内容：(i) 样本、阳性与阴性对照的原始测量值；(ii) 用于推导细菌蛋白生产速率的计算步骤；(iii) 计算中涉及的所有变量说明。 元数据表： 提供关联各数据集样本标识符的元数据文件（Metadata.csv），其中包含样本名称、提交至欧洲核苷酸档案库的测序读段文件名（.fastq）以及对应流式细胞术（.FCS）文件之间的对应关系。各列的详细说明见Metadata_README.txt。 水流数据： 水流数据（单位：立方米每秒，m³ s⁻¹）源自瑞典气象与水文研究所的Vattenwebb服务，通过S-HYPE水文模型获取。数据为2022年SUBID 8110（锡格福拉松湖）子流域的日尺度数据。 补充文件S1-S3： 本系列补充文件展示了基于DNA与RNA测序数据集的溪流与湖泊群落（按细菌门或OTU层级）的相对丰度模式。