H3K36me3 protects the mouse epigenome from single nucleotide variations [mRNA-Seq]. Mus musculus

The goals of this project are to study the transcriptome profiling (RNA-seq) of mouse embryonic stem cells by histone mutants incorporation (H3.1K3M, H3.1K36M vs H3.1wt) or shRNA-mediated knockdown of methyl-transferase Suv39h1/h2 (shSuv39h1, shSuv39h2 vs shNC) Overall design: mRNA profiles of mES stable cell lines with overexpression of H3.1WT, H3.1K9M, H3.1K36M and shNC, shSuv39h1, shSuv39h2 were generated by deep sequencing, in double, using Illumina next-generation sequencing.

本项目的研究目标为：通过组蛋白突变体外源整合（H3.1K3M、H3.1K36M与野生型H3.1（H3.1wt）对照），或短发夹RNA（shRNA）介导的甲基转移酶Suv39h1/h2基因敲低（shSuv39h1、shSuv39h2与阴性对照shNC对照），开展小鼠胚胎干细胞（mouse embryonic stem cells, mES）的转录组分析（RNA-seq）研究。实验设计：采用Illumina下一代测序技术进行双端深度测序，获取过表达H3.1WT、H3.1K9M、H3.1K36M，以及转染shNC、shSuv39h1、shSuv39h2的稳定mES系的mRNA表达谱。