Gene expression alterations in primary choroid plexus epithelial cells infected with JCPyV

The human polyomavirus, JCPyV, establishes a lifelong persistent infection in the peripheral organs of a majority of the human population worldwide. Patients who are immunocompromised due to underlying infections, cancer, or to immunomodulatory treatments for autoimmune disease are at risk for developing progressive multifocal leukoencephalopathy (PML) when the virus invades the CNS and infects macroglial cells in the brain parenchyma. It is not yet known how the virus enters the CNS to cause disease. The blood-choroid plexus barrier is a potential site of virus invasion as the cells that make up this barrier are known to be infected with virus both in vivo and in vitro. To understand the effects of virus infection on these cells we challenged primary choroid plexus epithelial cells with JCPyV and profiled changes in host gene expression. We found that JC infection induced the expression of proinflammatory chemokines and downregulated junctional proteins essential for maintaining blood-CSF and blood-brain barrier function. These data contribute to our understanding of how virus infection of the choroid plexus can modulate the host cell response to neuroinvasive pathogens. Gene expression profiling analysis of primary human choroid plexus epithelial cells comparing in triplicate cells infected with human polyomavirus JCPyV with control uninfected cells, 7 days post infection. 3 replicates per condition, 6 overall total samples.

JC多瘤病毒（JCPyV）可在全球绝大多数人群的外周器官中建立终身持续性感染。因潜在感染、癌症或自身免疫病免疫调节治疗导致免疫功能低下的患者，当病毒侵入中枢神经系统（CNS）并感染脑实质中的大胶质细胞时，罹患进展性多灶性白质脑病（PML）的风险显著升高。目前尚不明确该病毒是如何侵入中枢神经系统引发疾病的。血-脉络丛屏障是病毒侵入的潜在位点，已有研究证实构成该屏障的细胞在体内及体外均能被该病毒感染。为探究病毒感染对这些细胞的影响，我们使用JCPyV感染原代人脉络丛上皮细胞，并对宿主基因表达的变化进行了分析。研究发现，JCPyV感染可上调促炎趋化因子的表达，并下调维持血-脑脊液屏障与血脑屏障功能所必需的连接蛋白。本研究数据有助于加深我们对脉络丛病毒感染如何调控宿主细胞针对神经侵袭性病原体的应答的理解。本数据集为原代人脉络丛上皮细胞的基因表达谱分析，比较了感染JC多瘤病毒（JCPyV）的细胞与未感染的对照细胞，取样时间为感染后7天，每组设置3次生物学重复，总计6个样本。