Nonalcoholic fatty liver disease in AxB mice

Spontaneous insulin resistance and NAFLD emerged in AxB F1 male mice with parent-of-origin effects such that AB6F1 (AJ dam x B6 sire) were susceptible whereas B6AF1 (B6 dam x AJ sire) were resistant. We used microarrays to correlate gene expression with the NAFLD phenotype in 9-month-old male AB6F1 (affected) versus B6AF1 (unaffected) mice. Whole liver slices from two 9-month-old AB6F1 and B6AF1 males were collected for RNA extraction and hybridization on Affymetrix microarrays.

本研究中，AxB系杂交F1代雄性小鼠可自发出现胰岛素抵抗与非酒精性脂肪性肝病（NAFLD, Non-Alcoholic Fatty Liver Disease），且该表型存在亲本起源效应：AB6F1（AJ母鼠×B6公鼠）易感该疾病，而B6AF1（B6母鼠×AJ公鼠）则表现为抵抗状态。我们采用基因芯片（microarrays）技术，将9月龄雄性AB6F1（患病组）与B6AF1（健康对照组）小鼠的基因表达水平与NAFLD表型进行关联分析。本研究采集2只9月龄AB6F1与B6AF1雄性小鼠的全肝组织切片，用于RNA提取，并在Affymetrix基因芯片上完成杂交实验。