SH1870959.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为基于DNA的物种假说（Species Hypotheses，以下简称SH）的界定、识别、传播与相关研究提供了统一的规范化路径。国际核苷酸序列数据库中的所有真菌ITS序列，均通过应用一组动态距离阈值（0.5%~3.0%）完成聚类，聚类精度大致可达物种级别。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）形式存在的唯一且稳定的名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成审核验证。通过整合每个SH内所有序列的鉴定结果，尽可能为其匹配对应的分类单元名称及分类层级（如门、纲、目等）。研究人员会选取一条经自动或人工指定的序列作为该SH的代表序列。此类代表序列已发布于（https://unite.ut.ee/repository.php），可供科学界用于本地序列相似性搜索、高通量测序（next-generation sequencing）分析流程等研究场景。随着公共真菌ITS序列数量的持续增长，该系统与数据集将自动完成更新。