The Genetic Basis of Rapidly Evolving Male Genital Morphology in Drosophila.. Drosophila melanogaster

We performed genome-wide expression assays comparing gene expression in the Drosophila melanogaster third larval instar genital imaginal disc between males and females. We used microarrays to compare the relative expression levels of five independent male versus female comparisons for each of two different D. melanogaster wild-type strains, Canton-S and Berlin. Overall design: All microarrays were dual channel direct comparisons of late third larval instar male versus female genital imaginal discs from two Drosophila melanogaster wildtype strains, Canton S and Berlin. For each strain five independent biological samples were analyzed using a dye-swap design (i.e. male samples labeled with Cy5 in three replicates were labeled with Cy3 in the other two replicates in that experimental set).

我们开展了全基因组表达分析实验，比较黑腹果蝇（Drosophila melanogaster）三龄幼虫期生殖成虫盘的雌雄基因表达差异。本研究采用基因微阵列（microarrays），针对两种不同的黑腹果蝇野生型品系——Canton-S与Berlin，各设置5组独立的雌雄比对实验以分析其相对表达水平。实验整体设计：所有基因微阵列实验均采用双通道直接比对方案，比对样本为两种黑腹果蝇野生型品系（Canton S与Berlin）的晚期三龄幼虫期雌雄生殖成虫盘。针对每个品系，我们采用染料互换（dye-swap）实验设计分析5份独立生物学样本：在该实验组中，3次重复实验的雄性样本以Cy5标记，剩余2次重复实验则将雄性样本以Cy3标记。