Caenorhabditis sex-specific RNAseq

This project defines the transcriptomes of XO (male) and XX (female or mutant pseudo-female) Caenorhabditis nematodes. The data allow the overall composition and sexual regulation of the transcriptome within a single species to be determined. In addition, the five related species studied allow meta-comparisons between them. Because two of the five (C. elegans and C. briggsae) produce a self-fertile XX hermaphrodite, while the XX sex in the remaining three (C. japonica, C. remanei, and C. brenneri) are true females, the data are particularly useful for inferring effects of sexual mode on genome-wide gene expression. Overall design: L4 larvae and adults were pooled for each sex for five species (C. elegans, C. briggsae, C. japonica, C. brenneri, and C. remanei). Each of these 10 species-sex combinations was replicated three times, for a total of 30 samples.

本项目完成了隐杆线虫属（Caenorhabditis）内XO型（雄性）与XX型（雌性或突变型假雌性）线虫的转录组（transcriptome）解析工作。该数据集可用于解析单一物种内转录组的整体构成与性别调控机制。此外，本研究涉及的5个近缘物种可用于跨物种元比较分析。由于5个研究物种中，秀丽隐杆线虫（C. elegans）与布氏隐杆线虫（C. briggsae）可产生可自我受精的XX型雌雄同体，而其余3个物种——日本隐杆线虫（C. japonica）、雷曼隐杆线虫（C. remanei）与布伦纳隐杆线虫（C. brenneri）的XX个体均为真正的雌性，因此该数据集尤其适用于推断繁殖模式对全基因组基因表达的影响。整体实验设计如下：针对上述5个物种，分别按性别混合收集L4期幼虫与成虫样本；这10组物种-性别组合均设置3次生物学重复，最终共计获得30个样本。