Data underlying the publication: Interfacial protein adsorption behavior can be connected across a wide range of timescales using the microfluidic EDGE tensiometer

# IntroductionIn the current study, we expand the use of our EDGE (Edge-based Droplet GEneration) device, and validate the findings with ADT (automated drop tensiometer) results at complementary timescales.Furthermore, we explore if the EDGE tensiometer could distinguish effects occurring at high whey protein concentrations (2.5 – 10 wt%).In this dataset, you can find an excel file with an overview of all dataset named "EDGEvsADT_WPI_20240516_v09_final.xlxs" (in the Analyzed_Data folder).You can also find the raw data in the Raw_Data folder with .txt files.Finally, in the Plotted_Data_Scripts folder you can find all processed data from the excel file in .txt extension together with a Matlab code to plot the data.<br># AbstractHypothesisOur hypothesis is that dynamic interfacial tension values as measured by the partitioned-Edge-based Droplet GEneration (EDGE) tensiometry can be connected to those obtained with classical techniques, such as the automated drop tensiometer (ADT), expanding the range of timescales towards very short ones.<br>ExperimentsOil-water and air-water interfaces are studied, with whey protein isolate solutions (WPI, 2.5 – 10 wt%) as the continuous phase.The dispersed phase consists of pure n-hexadecane or air. The EDGE tensiometer and ADT are used to measure the interfacial (surface) tension at various timescales.A comparative assessment is carried out to identify differences between protein concentrations as well as between oil-water and air-water interfaces.<br>FindingsThe EDGE tensiometer can measure at timescales down to a few milliseconds and up to around 10 seconds, while the ADT provides dynamic interfacial tension values after at least one second from droplet injection and typically is used to also cover hours.The interfacial tension values measured with both techniques exhibit overlap, implying that the techniques provide consistent and complementary information.Unlike the ADT, the EDGE tensiometer distinguishes differences in protein adsorption dynamics at protein concentrations as high as 10 wt% (which is the highest concentration tested) at both oil-water and air-water interfaces.<br># Measurements and data collectionMeasurements and data collection were done according to what is described in the paperWe used two main setups and equipment, namely ADT (Automated Drop Tensiometer) and EDGE (partitioned-Edge-based Droplet GEneration) for data collection

# 引言
本研究拓展了EDGE（Edge-based Droplet GEneration，基于边缘的液滴生成）装置的应用范围，并通过互补时间尺度下的ADT（automated drop tensiometer，自动滴形张力仪）结果对研究发现进行验证。此外，本研究探究EDGE张力仪能否区分高乳清蛋白浓度（2.5~10 wt%）下出现的相关效应。
本数据集的分析数据汇总于Analyzed_Data文件夹下的"EDGEvsADT_WPI_20240516_v09_final.xlxs"Excel文件中；原始数据存放在Raw_Data文件夹内，格式为.txt文件；此外，Plotted_Data_Scripts文件夹中包含了从上述Excel文件导出的所有处理后.txt格式数据，以及用于绘图的Matlab代码。

# 摘要
## 假设
我们的假设为：通过分区式EDGE（partitioned-Edge-based Droplet GEneration，基于边缘的液滴生成）张力法测得的动态界面张力值，可与经典技术（如自动滴形张力仪ADT）获得的结果相关联，从而将可测量的时间尺度拓展至极短区间。

## 实验
本研究探究了油-水界面与气-水界面，以乳清分离蛋白（Whey Protein Isolate，WPI）溶液（2.5~10 wt%）作为连续相，分散相为正十六烷纯品或空气。采用EDGE张力仪与ADT在不同时间尺度下测量界面（表面）张力，并开展对比评估，以区分不同蛋白浓度以及油-水与气-水界面之间的差异。

## 研究结果
EDGE张力仪可在数毫秒至约10秒的时间尺度下完成测量；而ADT则可在液滴注入至少1秒后测得动态界面张力值，通常还可覆盖至小时级别的时间尺度。两种技术测得的界面张力值存在重叠区间，表明二者可提供一致且互补的实验数据。与ADT不同的是，EDGE张力仪能够在油-水与气-水两种界面下，区分最高至10 wt%（本研究测试的最高浓度）的蛋白浓度下的蛋白吸附动力学差异。

# 测量与数据采集
测量与数据采集流程遵循本论文所述规范。本研究主要采用两套实验装置与设备完成数据采集，分别为ADT（Automated Drop Tensiometer，自动滴形张力仪）与EDGE（partitioned-Edge-based Droplet GEneration，分区式基于边缘的液滴生成装置）。