Ascending Neurons 2023 split-GAL4 lines

This data set consists of central nervous system fluorescence images of Drosophila melanogaster driver lines and their genotypes. It includes raw confocal image data in Zeiss LSM format, along with H5J-compressed alignments to JRC2018, movies, maximum intensity projections (MIPs), and color-depth-encoded maximum intensity projections (CDMs). <br> Split-GAL4 AD and DBD combinations were designed by RM &amp; BD and screened in initial split combinations crossed by Janelia's Fly Facility, and dissected, antibody labeled, and imaged by FlyLight. Images were scored by RM and successful AD/DBD combinations were made into stable lines by the Fly Facility and imaged again by FlyLight. Full genotypes are listed for each line. <br> These split-GAL4 lines were used in support of https://doi.org/10.1038/s41593-023-01281-z . In association with that work, other processed and aligned FlyLight images were made available at https://doi.org/10.7910/DVN/S8AMKU .

本数据集涵盖黑腹果蝇（Drosophila melanogaster）驱动品系及其基因型的中枢神经系统荧光成像数据。其中包含Zeiss LSM格式的原始共聚焦成像数据，以及适配JRC2018模板的H5J压缩对齐图像、影像序列、最大强度投影图（maximum intensity projections, MIPs）与色彩深度编码最大强度投影图（color-depth-encoded maximum intensity projections, CDMs）。
Split-GAL4的AD与DBD组合由RM与BD设计，初始拆分组合经Janelia的果蝇设施完成杂交筛选，后续由FlyLight团队完成样品解剖、抗体标记及成像。成像结果由RM进行评估筛选，符合要求的AD/DBD组合由该果蝇设施构建为稳定品系，并再次由FlyLight团队完成成像。每个品系均附带完整基因型列表。
本批Split-GAL4品系被用于支撑论文https://doi.org/10.1038/s41593-023-01281-z 的相关研究工作。与该研究配套的其他经处理与对齐的FlyLight成像数据，已通过https://doi.org/10.7910/DVN/S8AMKU 公开获取。