Proximal grain composition of 240 globally diverse chickpea lines

The proximal composition (starch, protien, soluble sugars, lipid, insoluble fiber, and moisture) of 240 varieties of chickpea (Cicer arietinum) sourced from the ICRISAT genebank. \nData is linked to the publication "GWAS of chickpea grain macronutrient and lipidomic profiles from a global diversity panel" Buck et. al. 2025\nLineage: Chickpea grain was grown under common glasshouse conditions then de-hulled and ground into flour for analysis. \nProtein was measured by bradford assay.\nSoluble sugars were extracted by 80% ethanol and measured by anthrone. \nStarch was measured on sugar extracted pellet through digestion and measurement with GOPOD according to a megazyme kit. \nThe remaining pellet was digested with proteinase K, washed and dried to yield total insoluble fibre. \nLipids were extracted by chloroform:methanol, with phases separated by ammonium acetate, extracts were dried and weighed. \nMoisture was measured by drying flours at 40 degrees for a week. \nAll samples were measured in triplicate. Values presented are the mean and SE of three technical replicates.\nRegion of Origin classifications are based on Igolkina et al. 2023

本数据集包含从国际半干旱热带作物研究所（ICRISAT）种质库获取的240份鹰嘴豆（Cicer arietinum）样品的常规营养组成，涵盖淀粉、蛋白质、可溶性糖、脂质、不溶性膳食纤维及水分。
本数据集关联发表于2025年的论文《基于全球多样性群体的鹰嘴豆籽粒宏量营养素与脂质组全基因组关联分析》（"GWAS of chickpea grain macronutrient and lipidomic profiles from a global diversity panel"），作者为Buck等人。
样品制备流程：鹰嘴豆籽粒在标准化温室条件下种植，随后脱壳并磨制成面粉用于后续分析。
蛋白质采用Bradford检测法（Bradford assay）进行定量。
可溶性糖经80%乙醇提取后，采用蒽酮法完成定量检测。
淀粉含量以经糖提取后的沉淀为样本，通过酶解结合GOPOD检测法，并参照Megazyme试剂盒的操作流程进行测定。
剩余沉淀经蛋白酶K酶解、洗涤及干燥后，即可得到总不溶性膳食纤维。
脂质采用氯仿-甲醇混合溶剂提取，经乙酸铵溶液分离两相后，将提取液干燥并称重以实现定量。
水分含量通过将面粉样品置于40℃环境中干燥一周后称重计算得到。
所有样品均进行三次技术重复测定，报告结果为三次重复的平均值与标准误（SE）。
样品的起源地分类参照Igolkina等人2023年的研究成果。