Transcriptome analysis of wildtype elmo3+/+ and homozygous elmo3-/- knockout zebrafish larvae at 120 hpf through next generation RNA sequencing [dataset 3]. Transcriptome analysis of wildtype elmo3+/+ and homozygous elmo3-/- knockout zebrafish larvae at 120 hpf through next generation RNA sequencing [dataset 3]

Elmo3 is one member of the Elmo protein family. In our study we addressed the question if the functions of Elmo1, Elmo2 and Elmo3 ware consistent or if they differ. With the CRISPR/Cas9 system we generated single knockout mutants of elmo1, elmo2 and elmo3 in the zebrafish. To assess the impact of the loss of one of the proteins on the transcriptome of a developing organism, we did RNA sequencing and transcriptome analysis with elmo1+/+ and elmo1-/-, elmo2+/+ and elmo2-/- and elmo3+/+ and elmo3-/- zebrafish larvae at 120 hours post fertilization. Here we provide the sequencing data of elmo3+/+ and elmo3-/-. Overall design: Total RNA profiles of wildtype and homozygous elmo3 knockout zebrafish larvae samples

Elmo3是Elmo蛋白家族的成员之一。本研究旨在探究Elmo1、Elmo2与Elmo3的功能是否一致，抑或存在差异。我们利用CRISPR/Cas9系统，在斑马鱼中构建了elmo1、elmo2及elmo3的单基因敲除突变体。为评估单一蛋白缺失对发育中生物体转录组的影响，我们对受精后120小时的elmo1+/+与elmo1-/-、elmo2+/+与elmo2-/-、elmo3+/+与elmo3-/-斑马鱼幼虫进行了RNA测序及转录组分析。本研究提供elmo3+/+与elmo3-/-样本的测序数据。整体实验设计：野生型及纯合elmo3敲除斑马鱼幼虫样本的总RNA谱