Identification of novel human intermediate progenitor genes in cortical development

To identify the transcriptional signature of human IPCs (hIPCs), we isolated TBR2-positive IPCs from developing human neocortex. We utilized an antibody to mark intranuclear TBR2 in single-nucleus suspensions purified from developing human neocortex procured at the peak of neurogenesis at gestational weeks (GW)14-17, and sorted the TBR2+ nuclei (considered hIPC derived) from the TBR2- nuclei (considered non-hIPC derived). RNA-seq was performed to determine the expression profiles of hIPC-enriched genes.

为鉴定人类IPC（hIPCs）的转录特征，我们从发育中的人类新皮层中分离出TBR2阳性IPC。我们使用抗体标记采自神经发生高峰期（妊娠14-17周，GW14-17）的发育人类新皮层纯化所得的单细胞核悬液中的核内TBR2，并将TBR2阳性细胞核（认定为hIPC来源）与TBR2阴性细胞核（认定为非hIPC来源）进行分选。随后通过RNA测序（RNA-seq）分析，以确定hIPC富集基因的表达谱。