Additional file 3 of A comparative study on normal and obese mice indicates that the secretome of mesenchymal stromal cells is influenced by tissue environment and physiopathological conditions

Additional file 2. List of proteins identified in MSC secretome. “ND HFD tech biol replicates” spreadsheet: The sheet shows the list of proteins found in vWAT-MSCs, sWAT-MSCs, and BM-MSCs isolated from samples taken from ND-treated mice designated as 1, 2, and 3 and from HFD-treated mice designated as 4, 5, and 6. For each biological sample, there were two technical replicates (A, B). Proteins were listed with their UniProt identifiers. “ND HFD common data” spreadsheet: The proteins secreted by vWAT-MSCs isolated from samples taken from mouse 1, 2, and 3 were analyzed with a Venn graph to find common data. The procedure was also performed for sWAT-MSCs and BM-MSCs. The sheet also lists proteins isolated from samples taken from mice 4, 5, and 6, which were analyzed with the same method. “Venn comparison in ND or HFD” spreadsheet: The sheet shows the result of Venn diagram comparison among vWAT-MSCs, sWAT-MSCs, and BM-MSCs coming from ND- and HFD-treated mice. “Venn comparison in ND vs. HFD” spreadsheet: The sheet shows the result of Venn diagram comparison of vWAT-MSCs from ND-treated mice versus vWAT-MSCs from HFD-treated mice. The same procedure was employed for sWAT-MSCs and BM-MSCs.

附加文件2：间充质干细胞（MSC）分泌组鉴定蛋白列表。「ND HFD 技术重复与生物学重复」工作表：本工作表展示了从正常饮食（ND）处理组小鼠（编号1、2、3）与高脂饮食（HFD）处理组小鼠（编号4、5、6）中分离得到的内脏白色脂肪组织间充质干细胞（vWAT-MSCs）、皮下白色脂肪组织间充质干细胞（sWAT-MSCs）及骨髓间充质干细胞（BM-MSCs）的检测蛋白列表。每份生物学样本设置2个技术重复（A、B），蛋白以其UniProt标识符进行标注。「ND HFD 公共蛋白数据」工作表：对从编号1、2、3的ND处理组小鼠中分离得到的vWAT-MSCs分泌蛋白进行韦恩图分析以筛选公共蛋白数据，该分析流程同样适用于sWAT-MSCs与BM-MSCs。本工作表同时列出了编号4、5、6的HFD处理组小鼠样本分离得到的蛋白，并采用相同方法完成分析。「ND或HFD组内韦恩比较」工作表：本工作表展示了ND处理组与HFD处理组来源的vWAT-MSCs、sWAT-MSCs及BM-MSCs之间的韦恩图比较结果。「ND组与HFD组韦恩对比」工作表：本工作表展示了ND处理组vWAT-MSCs与HFD处理组vWAT-MSCs的韦恩图比较结果，该分析流程同样应用于sWAT-MSCs与BM-MSCs。