Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle

Global warming is a major challenge to the sustainable and humane production of food because of the increased risk of livestock to heat stress. Here, the example of the prolactin receptor (PRLR) gene is used to demonstrate how gene editing can increase the resistance of cattle to heat stress by the introduction of mutations conferring thermotolerance. Several cattle populations in South and Central America possess natural mutations in PRLR that result in affected animals having short hair and being thermotolerant. CRISPR/Cas9 technology was used to introduce variants of PRLR in two thermosensitive breeds of cattle – Angus and Jersey. Gene-edited animals exhibited superior ability to regulate vaginal temperature (heifers) and rectal temperature (bulls) compared to animals that were not gene-edited. Moreover, gene-edited animals exhibited superior growth characteristics. There was no evidence for deleterious effects of the mutation on carcass characteristics or male reproductive function...., , , # Data from: Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle

[https://doi.org/10.5061/dryad.8sf7m0cwj](https://doi.org/10.5061/dryad.8sf7m0cwj)

## Description of the data and file structure

All details of data including units are part of the accompanying Excel file. The exception is for CASA data, where units can be found in the accompanying publication. Note that entries with \".\" are missing values.

全球变暖会提升家畜热应激风险，对粮食的可持续与人道化生产构成重大挑战。本研究以催乳素受体（prolactin receptor, PRLR）基因为例，阐明如何通过引入赋予耐热性的突变，借助基因编辑技术增强牛的热应激抵抗能力。南美与中美洲的多个牛群天然携带PRLR突变，携带该突变的个体被毛较短且具备耐热性。本研究采用CRISPR/Cas9技术，在安格斯（Angus）与泽西（Jersey）这两个热敏性牛品种中引入PRLR突变变体。与未经过基因编辑的对照牛只相比，基因编辑牛的体温调节能力更优：青年母牛（heifers）的阴道温度调控效果更佳，公牛（bulls）的直肠温度调控效果更优。此外，基因编辑牛的生长性能更优异。未发现该突变对牛只胴体性状或雄性生殖功能产生有害影响的证据。……
# 数据来源：《催乳素受体基因编辑对牛耐热性、生长性能及雄性生殖功能的影响》
[https://doi.org/10.5061/dryad.8sf7m0cwj](https://doi.org/10.5061/dryad.8sf7m0cwj)

## 数据与文件结构说明
所有数据细节（包括单位）均收录于随附的Excel文件中。仅计算机辅助精液分析（Computer-Aided Semen Analysis, CASA）数据的单位需查阅随附的已发表文献。请注意，以“.”标注的条目为缺失值。