Transcriptional analysis of AHLs signal treatment at 30°C. Yersinia pestis

Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of AHL quorum sensing was investigated by comparing transcript profiles when two AHL quorum-sensing signals are added in. The strain Δpgm (pigmentation-negative) mutant was called wild type. The two AHLs signals are N-(3-Oxooctanoyl)-L-homoserine lactone and N-Hexanoyl-DL-homoserine lactone.The control consisted of cells grown and treated under the same conditions without added signals. Overall design: Six independent RNA samples from Y. pestis CO92 Δpgm cultures were paired with six independent RNA samples from two AHLs added cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

鼠疫耶尔森菌(Yersinia pestis)是鼠疫的致病原，可通过群体感应(quorum sensing)感知细胞密度。目前鼠疫耶尔森菌中群体感应的功能尚不明确。本研究通过对比添加两种酰基高丝氨酸内酯(AHL)群体感应信号后的转录组谱，探究了该菌的AHL群体感应过程。本研究将Δpgm（色素阴性）突变株定义为野生型菌株。两种AHL信号分别为N-(3-氧代辛酰基)-L-高丝氨酸内酯与N-己酰基-DL-高丝氨酸内酯。对照组为在相同条件下培养并处理、未添加任何信号分子的菌体。
实验整体设计：将6份取自鼠疫耶尔森菌CO92 Δpgm培养物的独立RNA样本，与6份取自添加了两种AHL的培养物的独立RNA样本进行配对，用于6张双色微阵列的杂交实验。采用染料互换(dye-swap)实验设计以消除Cy5与Cy3荧光染料的偏倚。