Mass spectrometry identification of proteins in spots up-regulated in or unique to MCF7 as compared to T47D cell line.

The function description and/or biological process and the subcellular location were from the UniProt database (www.uniprot.org). Spot, spot number; FC, fold change; MW, molecular weight; pI exp, isoelectric point as determined from the 2-D gel experiments; Pep, number of unique peptides; U, unique. The number after the protein name indicated the additional spot in which the protein was found.
‡Protein previously reported to be used as a breast cancer biomarker and which were overexpressed in cancerous cells based on data from the literature [9], [10].
*Protein used for Q-RT-PCR validation.

本研究涉及的蛋白质功能描述、生物学过程及亚细胞定位信息（或其中任意组合）均来源于通用蛋白质知识库（UniProt，www.uniprot.org）。斑点（Spot）：斑点编号；折叠变化（fold change，FC）：表达水平的折叠变化；分子量（molecular weight，MW）：蛋白质的分子量；pI exp：指通过二维凝胶电泳实验测定的等电点；Pep：独特肽段的数量；U（unique）：代表独特性。蛋白质名称后的数字表示该蛋白被检出的额外斑点序号。
‡ 基于文献[9]、[10]的数据，该蛋白此前被报道可作为乳腺癌生物标志物，且在癌细胞中呈过表达状态。
* 该蛋白用于定量逆转录PCR（Q-RT-PCR）验证。