Transcriptomic insight into differential gene expression patterns of the camel pathogenic nematode, Parabronema skrjabini, at specific developmental stages. Parabronema skrjabini

Parabronema skrjabini is an widespread but neglected blood-feeding nematode. The P.skrjabini can ,as a pathogen ,causes the large ruminants clinical and pathological effects, with even severe infections being fatal, which is of a major veterinary importance in many developing countries. However, there is little information that controls the developmental changes of the parasite survived and developed in insect and ruminant hosts. Here we have collected P.sktjabini of four specific developmental stages and allows for complete understanding the key characteristics of development. We analyzed the transcriptomic changes accompanying the four specific developmental stages using RNA-seq and pinpointed the development-dependent function genes and biological processes. Overall design: P.skrjabini were collected at four different developmental stages (egg, larva, adult female,adult male ) for RNA-seq.

斯氏副柔线虫（Parabronema skrjabini）是一种分布广泛却被忽视的吸血线虫。该寄生虫作为病原，可引发大型反刍动物出现临床与病理症状，重度感染甚至可导致宿主死亡，在众多发展中国家具有重要的兽医学意义。然而，目前尚缺乏关于该寄生虫在昆虫与反刍动物宿主体内存活与发育过程中相关调控机制的研究资料。本研究收集了四个特定发育阶段的斯氏副柔线虫，以期全面解析其发育关键特征。本研究通过RNA测序（RNA-seq）技术，分析了这四个特定发育阶段的转录组变化，精准定位了与发育相关的功能基因及生物学过程。实验设计概述：为开展RNA-seq分析，本研究采集了斯氏副柔线虫的四个不同发育阶段样本（虫卵、幼虫、雌性成虫、雄性成虫）。