Underlying and Extended Data for Refined Home-Brew Media for Cost-Effective, Weekend-Free hiPSC Culture and Genetic Engineering

Extended Data and raw data for the manuscript "Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering"

Extended Data 1 - Images of WTC11 cells adapted to weekend-free media or commercial E8 taken 24, 48 and 72 hours after passage. Contains raw .tiff files for each image and a .pdf with a compiled figure

Extended Data 2 - Fold change and adj.  p-values from the statistical analyses of RNA-seq data of the cells grown in weekend-free media and commercial E8

Extended Data 3 - Biological Process GO terms for genes upregulated in cells grown in B8+ and cE8

Extended Data 4 - SOP for preparation of the supplement for hE8 and B8+ weekend-free media

Extended Data 5 - Gene counts, supporting files, and output results of RNA-seq analyses relative to GitHub repository https://github.com/alessandro-bertero/BulkRNAseq_media

Manuscript_data - Raw data used for generating Figures 1,2 and 4 in the manuscript.

Bulk_RNA_seq_archive - archived source code from https://github.com/alessandro-bertero/BulkRNAseq_media used to generate results in Figure 3.

论文《适配低成本高效、无需周末值守的诱导多能干细胞（hiPSC）培养与基因工程的优化自制培养基》的补充数据与原始数据。
补充数据集1：传代后24、48及72小时拍摄的、适配无需周末值守培养基或商业化E8培养基的WTC11细胞图像。包含每张图像的原始.tiff文件，以及一张汇总拼接图的.pdf文件。
补充数据集2：对在无需周末值守培养基与商业化E8培养基中培养的细胞进行RNA测序（RNA-seq）数据分析得到的倍数变化值与校正后p值。
补充数据集3：在B8+与cE8培养基中培养的细胞内上调基因的基因本体（GO）生物过程术语集。
补充数据集4：hE8与B8+无需周末值守培养基补剂的制备标准操作流程（SOP）。
补充数据集5：与GitHub仓库https://github.com/alessandro-bertero/BulkRNAseq_media相关的RNA测序分析的基因计数文件、辅助文件与输出结果。
手稿原始数据（Manuscript_data）：用于生成论文中图1、图2与图4的原始数据。
批量RNA测序归档数据集（Bulk_RNA_seq_archive）：用于生成论文图3结果的、源自https://github.com/alessandro-bertero/BulkRNAseq_media的归档源代码。