Raw Data_ZC 20241105.xlsx

We investigated Polyphosphate (polyP) cycling in a cross-latitudinal section (15°N-39°N, 150°E) of the Northwest Pacific Ocean, covering a diverse nutrient gradient and varying P stress conditions, 2023. This data set includes biogeochemical parameters as follows, polyP, Chl a, nutrients, alkaline phosphatase activity (APA), picophytoplankton, total particulate phosphorus (TPP). Detailed methods can be as follows,<br>We used a conductivity-temperature-depth (CTD) instrument cluster (Seabird 911) to collect seawater samplers.<br>Seawater samples were filtered onto GF/F filters to obtain polyP and TPP samplers. PolyP was quantified using a fluorescence method according to the protocol described by Martin et al., 2013, doi: 10.1128/AEM.02592-12. TPP was converted into phosphate and quantified using a UV spectrophotometer (Shi et al., 2019, doi: 10.5194/acp-19-847-2019).<br>APA was calculated through the change of fluorescence using a Turner Trilogy fluorometer, details can be seen in Jin et al., 2024, doi: 10.1038/s41467-024-44838-7.<br>Nutrient samplers were filtered through syringe filters (0.45 μm in core size) and determined by a QuAAtro Continuous-Flow Analyzer (SEAL Analytical) (Grasshoff et al., 1999, Methods of seawater analysis).<br>Chl a samplers were filtered onto Whatman GF/F filters (47 mm in diameter) and were determined using a Turner Trilogy fluorometer (Strickland &amp; Parsons, 1972, A Practical Handbook of Seawater Analysis).<br>Picophytoplankton was determined using a Beckman CytoFLEX Flow Cytometer (Wei et al., 2020, doi: 10.3354/ame01928).

本研究于2023年针对西北太平洋15°N-39°N、150°E的跨纬度断面开展了多聚磷酸盐（Polyphosphate, polyP）循环过程的调查，该区域涵盖了多样的营养盐梯度与不同程度的磷胁迫条件。本数据集包含如下生物地球化学参数：多聚磷酸盐（polyP）、叶绿素a（Chl a）、营养盐、碱性磷酸酶活性（alkaline phosphatase activity, APA）、微微型浮游植物、总颗粒态磷（total particulate phosphorus, TPP）。
详细实验方法如下：
1. 采用导电率-温度-深度（conductivity-temperature-depth, CTD）集成设备（Seabird 911）采集海水样品。
2. 将海水样品过滤至GF/F滤膜上，以获取多聚磷酸盐与总颗粒态磷样品。多聚磷酸盐采用荧光法定量，参照Martin等（2013）发表的实验方案（doi: 10.1128/AEM.02592-12）。总颗粒态磷先被转化为磷酸盐，再通过紫外分光光度计进行定量（Shi等，2019，doi: 10.5194/acp-19-847-2019）。
3. 碱性磷酸酶活性通过Turner Trilogy荧光计检测荧光变化进行计算，具体细节可参考Jin等（2024）的研究（doi: 10.1038/s41467-024-44838-7）。
4. 营养盐样品经孔径0.45 μm的针式过滤器过滤后，使用QuAAtro连续流动分析仪（SEAL Analytical）完成测定（Grasshoff等，1999，《Methods of seawater analysis》（海水分析方法））。
5. 叶绿素a样品被过滤至直径47 mm的Whatman GF/F滤膜上，采用Turner Trilogy荧光计进行测定（Strickland & Parsons，1972，《A Practical Handbook of Seawater Analysis》（海水分析实用手册））。
6. 微微型浮游植物采用Beckman CytoFLEX流式细胞仪进行测定（Wei等，2020，doi: 10.3354/ame01928）。