Gene expression in alveolar macrophages between bleomycin–treated wild-type and S1pr2-/- mice.

We compared mRNA expression in alveolar macrophages between bleomycin–treated wild-type and S1pr2-/- mice, using DNA microarray analysis. In S1pr2-/- macrophages, 398 genes showed decreases to less than 50% of the levels in wild-type macrophages. In contrast, 122 genes showed more than 2.0-fold increases in S1pr2-/- macrophages compared with wild-type macrophages. The downregulated genes in S1pr2-/- mice included the following potentially fibrosis–related genes: profibrotic cytokines, chemokines, and the markers characteristic of classically activated (M1) and alternatively activated (M2) macrophages. BALF cells from wild-type and S1pr2-/- mice on day 22 after bleomycin administration were collected and analyzed. Two independent experiments were performed.

本研究采用DNA微阵列分析（DNA microarray analysis），对比博莱霉素（bleomycin）处理的野生型（wild-type）小鼠与S1pr2基因缺失型（S1pr2-/-）小鼠的肺泡巨噬细胞（alveolar macrophages）中mRNA的表达水平。在S1pr2基因缺失型肺泡巨噬细胞中，共有398个基因的表达量较野生型小鼠肺泡巨噬细胞下降至50%以下；与之相对，有122个基因的表达量较野生型小鼠上调超过2.0倍。S1pr2基因缺失型小鼠的下调基因包含以下潜在纤维化相关基因：促纤维化细胞因子（profibrotic cytokines）、趋化因子（chemokines），以及经典活化型（M1）与替代性活化型（M2）巨噬细胞的特征标志物。本研究于博莱霉素给药后第22天，收集野生型与S1pr2基因缺失型小鼠的支气管肺泡灌洗液（bronchoalveolar lavage fluid, BALF）细胞并开展分析，共完成两次独立重复实验。