Targeted epigenetic modulation using a DNA-based histone deacetylase inhibitor enhances cardiomyogenesis in mouse embryonic stem cells (ChIP-Seq). Targeted epigenetic modulation using a DNA-based histone deacetylase inhibitor enhances cardiomyogenesis in mouse embryonic stem cells (ChIP-Seq)

To determine how SAHA-PIP G can trigger the expression of the mesendoderm-related genes in mouse ES cells, chromatin immunoprecipitation (ChIP-seq) analysis was carried out with an antibody against the histone H3 lysine 4 trimethylation (H3K4me3) using EBs harvested on day 0 Overall design: Examination of H3K4me3 in 2 embryonic body types that with/without 1 μM SAHA-PIP G treatment.

为探究SAHA-PIP G诱导小鼠ES细胞（mouse ES cells）中内胚层相关基因表达的作用机制，本研究使用于第0天收获的拟胚体（embryoid bodies, EBs），采用靶向组蛋白H3赖氨酸4三甲基化（H3K4me3）的抗体开展染色质免疫共沉淀测序（ChIP-seq）分析。
实验整体设计：对比检测经1 μM SAHA-PIP G处理与未处理的两种拟胚体样本中的H3K4me3修饰水平。