Single-cell Transcriptomes of RPE-specific AAV-GFP/AAV-PDGF-D mouse RPE-choroid complex

Purpose: PDGF-D is a potent angiogenic and immune-related factor. It has been demonstrated that PDGF-D play roles in the pathological process of ocular angiogenesis and degenerative diseases. In our previous study, we established a retinal pigment epithelial (RPE) cell-specifc AAV-PDGF-D/GFP mouse model to study PDGF-D's role in age-related macular degeneration (AMD). In this study, we utilized the single-cell transcriptiome seqeuncing technique to explore the cell types of immune cells attracted by PDGF-D overepxression using the same animal models, with emphases on the impact of PDGF-D overexpression on individual cell's behaviour. scRNA-seq of mouse RPE-choroid complex received subretina injection of RPE-specific AAV-GFP and AAV-PDGF-D , which were collected after 4-weeks injection of AAVs.

研究目的：PDGF-D（血小板衍生生长因子D）是一种强效血管生成与免疫相关因子。已有研究证实，PDGF-D参与眼部血管生成及退行性疾病的病理进程。本团队前期研究中，我们构建了视网膜色素上皮（retinal pigment epithelial, RPE）细胞特异性的腺相关病毒（adeno-associated virus, AAV）-PDGF-D/GFP小鼠模型，用于探究PDGF-D在年龄相关性黄斑变性（age-related macular degeneration, AMD）中的作用。本研究采用单细胞转录组测序技术，以该动物模型为研究载体，分析PDGF-D过表达所招募的免疫细胞类型，并重点解析PDGF-D过表达对单个细胞行为的影响。我们对经视网膜下注射RPE特异性AAV-GFP与AAV-PDGF-D的小鼠视网膜色素上皮-脉络膜复合体开展了单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），样本采集于AAV注射4周后。