Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells. Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells

Loss of Pten and inhibition of PI3K induced apoptosis of hematopoietic stem/progenitor cells upon endothelial to hematopoietic transition and surviving hematopoietic stem/progenitor cells committed to all blood lineages but displayed reduced stemness Overall design: AGMs (36hpf) or CHTs (5dpf) were dissected and dissociated, using FACS sorting HSPCs were isolated and single cell RNA seq was performed.

Pten缺失与PI3K抑制可在内皮向造血转化（endothelial to hematopoietic transition）过程中诱导造血干/祖细胞（hematopoietic stem/progenitor cells）发生凋亡；存活的造血干/祖细胞虽可定向分化为所有血液谱系，但干细胞特性（stemness）出现降低。实验整体设计：取36小时受精后（36hpf）的主动脉-性腺-中肾区（AGMs）或5天受精后（5dpf）的尾部造血组织（CHTs），经解剖分离并解离后，通过荧光激活细胞分选（FACS）分离造血干/祖细胞，随后进行单细胞RNA测序。