Temporal dissection of breast cancer brain metastases. Temporal dissection of breast cancer brain metastases

The goal of this study was to identify temporal changes to breast cancer cells as they adapt to the brain metastatic microenvironment. MDA-MB-231-tdTomato human breast cancer cells were injected into the carotid artery of Rag1-/- mice and dissected at 7 or 40 days post injection for RNA-sequencing. Overall design: 3 samples were analyzed at 7 days post injection, and 9 samples were analyzed at 40 days post injection

本研究旨在探究乳腺癌细胞适应脑转移微环境过程中的时序动态变化。将MDA-MB-231-tdTomato人乳腺癌细胞注射至Rag1-/-小鼠的颈动脉中，并分别于注射后7天和40天解剖取材，开展RNA测序（RNA-sequencing）。实验整体设计：注射后7天组共分析3个样本，注射后40天组共分析9个样本。