Specific activity (nmol·s<sup>−1</sup>·mg<sup>−1</sup>) of DadB and comparison with other HLDs.
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Group A, chlorinated or brominated alkanes longer than C3; Group B, chlorinated or brominated C2 and C3 alkanes; Group C, iodinated alkanes and halogenated cycloalkanes, alkenes, ethers, nitriles; Group D, other substrates used in activity assay of DadB. ND, no detectable activity. NA, no available activity. 2-Iodobutane is unstable and its spontaneous dehalogenation interferes the detection of iodine ion concentration seriously, so accurate activity to 2-iodobutane was not obtained. aThe activity data of these five HLDs were collected from the paper of Koudelakova et al. [18].
组别A:碳链长度大于C3的氯代烷烃与溴代烷烃;组别B:C2及C3的氯代烷烃与溴代烷烃;组别C:碘代烷烃以及卤代环烷烃、烯烃、醚类与腈类;组别D:用于DadB活性测定的其余底物。 ND:未检测到活性(no detectable activity);NA:无可用活性数据(no available activity)。 2-碘丁烷(2-Iodobutane)性质不稳定,其自发脱卤反应会严重干扰碘离子浓度的检测,因此无法获得针对2-碘丁烷的准确活性测定结果。 注:该五种卤代烷烃脱卤酶(HLD)的活性数据取自Koudelakova等人发表于文献[18]的研究。



