Metagenomes from the Bedford Basin and the Scotian shelf Metagenome. Metagenomes from the Bedford Basin and the Scotian shelf

The Bedford Basin forms the inner portion of the Halifax Harbour. Freshwater is supplied from both the Sackville River and additional runoff from the watershed. The Basin is connected to the Atlantic Ocean through a channel which is 10 km long, 400 m wide and 20 m deep (Li and Dickie 2001). Since 1992, the Bedford Institute of Oceanography (BIO) has carried out weekly monitoring at the Compass Buoy station, located at the deepest point of the basin (71 m); water samples are collected at depths of 1, 5, 10 and 60 metres.Similarly, the Atlantic Zone Monitoring Program (AZMP) program that was implemented in 1998, aims to collect and analyze the biological, chemical and physical field data from the continental slope and the deep waters of the northwestern Atlantic Ocean.The broad goals of both these programs can be described as follows:- To characterize and understand the causes of oceanic variability at the seasonal, inter-annual and decadal scales- To provide multidisciplinary data sets that can be used to establish relationships among the biological, chemical and physical variables- To provide adequate data to support the sound development of ocean activitiesIn cooperation with BIO and Doug Wallace (CERC.OCEAN), we are examining the microbial communities of the Bedford Basin and the Scotian shelf utilizing techniques such as flow cytometry, microscopy, and genetic analyses. The metagenomes submitted in this project have been sequenced from samples collected from these monitoring programs.

贝德福德盆地（Bedford Basin）为哈利法克斯港（Halifax Harbour）的内湾区域。其淡水补给源自萨克维尔河（Sackville River）与流域内的其他径流。该盆地通过一条长10千米、宽400米、水深20米的水道与大西洋相连（Li与Dickie，2001）。
自1992年起，加拿大大西洋海洋科学研究所（Bedford Institute of Oceanography, BIO）会在位于盆地最深处（71米）的罗盘浮标站（Compass Buoy）开展每周例行监测，分别在1米、5米、10米及60米水层采集水样。
于1998年启动的大西洋区域监测计划（Atlantic Zone Monitoring Program, AZMP），旨在采集并分析西北大西洋大陆坡与深水区的生物、化学及现场物理观测数据。
上述两项监测计划的总体目标如下：
- 刻画并解析海洋变异性在季节、年际及年代际尺度上的驱动成因；
- 提供多学科数据集，用于构建生物、化学与物理变量间的关联关系；
- 提供充足数据支撑海洋活动的稳健发展。
本项目与BIO及道格·华莱士（Doug Wallace, CERC.OCEAN）合作，采用流式细胞术（flow cytometry）、显微镜观测及遗传学分析等技术，对贝德福德盆地与斯科舍陆架（Scotian Shelf）的微生物群落展开研究。本项目提交的宏基因组（metagenomes）测序数据，均源自上述监测项目所采集的样本。