Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line. [sip53]. Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line. [sip53]

Mutant p53 can acquires oncogenic properties supporting tumor growth, metastases and chemoresistance, by reprogramming cancer cell transcriptome, proteome and metabolome. To investigate what is the gene expression network regulated by mutant p53, we silenced its expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MDA-MB 231 cells transfected with Control or p53 siRNAs, each in triplicate.

突变型p53（mutant p53）可通过重编程癌细胞的转录组、蛋白质组与代谢组，获得促进肿瘤生长、转移及化疗耐药的致癌特性。为探究突变型p53所调控的基因表达网络，我们在三阴性乳腺癌（Triple Negative Breast Cancer, TNBC）细胞系MDA-MB-231中沉默了p53的表达。随后，我们利用MDA-MB-231细胞的RNA测序（RNA-seq）数据开展基因表达谱分析。
整体设计：对转染对照小干扰RNA（small interfering RNA, siRNA）或p53小干扰RNA的MDA-MB-231细胞的RNA测序数据进行对比基因表达谱分析，每组均设置3次重复。