LPS-induced cochlear inflammation and SPT-2101 treatment

[Methods] ABR DATA: obtained by ABR recording in a Tucker David Technology RZ6 Auditory Workstation using BioSigRZ software EVANS BLUE DATA: obtained by fluorimetry in a Glomax??? Luminometer and Microplate Reader (Promega) (620/680 nm excitation/emission wavelength). GENE EXPRESSION DATA: obtained by Real-Time PCR and analyzed by QuantStudio??? Real-Time PCR software 1.3. PCR array obtained using the PCR array RT?? Profiler??? Rat Innate & Adaptive Immune Responses (Qiagen, #PARN-052Z) and analyzed with Geneglobe (Qiagen). MRI DATA: images obtained with a horizontal 7.0-Tesla Bruker BioSpec?? system (Bruker Medical GmbH) running ParaVision 6.0.1 on a Linux environment. Data analysis was performed with Fiji software. OPTICAL IMAGING DATA: images obtained with a Zeiss AxioPhot microscope (Carl Zeiss). Data analysis was performed with Fiji software. FLUORESCENCE DATA: images obtained with an epifluorescence (Nikon 90i) and confocal laser-scanning (Zeiss LSM710) microscopes. Data analysis was performed with Fiji software. WB DATA: Immunoreactive bands revealed using the Clarity??? Western ECL Substrate (Bio-Rad), and images were captured with the ImageQuant LAS4000 mini digital camera using ImageQuant TL software 8.1 (GE Healthcare).

【方法】
ABR数据：使用BioSigRZ软件，在塔克·戴维科技（Tucker David Technology）RZ6听觉工作站中通过听性脑干反应（ABR）记录获取。
伊文思蓝（EVANS BLUE）数据：采用Glomax？？？型发光光度计与微孔板检测仪（Promega公司）通过荧光光度法获取，激发/发射波长为620/680 nm。
基因表达数据：通过实时定量PCR获取，并使用QuantStudio？？？实时定量PCR软件1.3版进行分析；所用PCR芯片为Qiagen公司货号PARN-052Z的RT？？ Profiler？？大鼠天然免疫与适应性免疫应答芯片，并通过Qiagen的Geneglobe软件完成分析。
磁共振成像（MRI）数据：采用搭载Linux环境、运行ParaVision 6.0.1版本软件的布鲁克医疗（Bruker Medical GmbH）7.0 T水平式BioSpec？？？系统采集图像，数据分析采用Fiji软件完成。
光学成像数据：使用蔡司AxioPhot显微镜（卡尔·蔡司，Carl Zeiss）采集图像，数据分析采用Fiji软件完成。
荧光成像数据：使用落射荧光显微镜（尼康90i，Nikon 90i）与共聚焦激光扫描显微镜（蔡司LSM710，Zeiss LSM710）采集图像，数据分析采用Fiji软件完成。
蛋白质免疫印迹（WB）数据：采用Bio-Rad公司的Clarity？？？Western ECL底物显影免疫反应条带，使用通用电气医疗（GE Healthcare）公司的ImageQuant LAS4000 mini数码相机搭配ImageQuant TL 8.1版软件捕获图像。