Intercropping with legume induces disease suppression of banana Fusarium wilt

We conducted a field experiment to assess microbiome shifts and resulting outcomes of banana Fusarium wilt as induced by intercropping banana with the legume Trifolium repens on. We further conducted a pot experiment to examine the impacts of the legume crop on microbiome across different spatial distances. Bacterial-, fungal-, and eukaryote-universal primers sets were used for high-throughput Illumina MiSeq sequencing.本研究开展田间试验，以评估香蕉与豆科作物白三叶（Trifolium repens）间作诱导的微生物组（microbiome）群落变迁，以及由此引发的香蕉镰刀菌枯萎病（banana Fusarium wilt）发生状况。此外，本研究还设置盆栽试验，探究该豆科作物在不同空间距离下对微生物组的影响。实验采用细菌、真菌及真核生物通用引物（universal primers）进行高通量Illumina MiSeq测序。