ExtFig3A_RACE_BCAP31_R3_LG293.sgd
收藏DataCite Commons2023-01-19 更新2024-08-18 收录
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https://figshare.com/articles/dataset/ExtFig3A_RACE_BCAP31_R3_LG293_sgd/21804516
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<em>Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nucleotides downstream of the predicted cut sites.The PCR products were run on an agarose gel. </em>
将Xrn1基因敲除的A549细胞分别感染野生型PR8（WT PR8）、PR8-PA(ΔX)毒株，或进行Mock空白感染。随后使用针对BCAP31、TUBA1B、INSIG1及SLC7A5的特异性引物开展5'快速扩增cDNA末端（5' RACE）实验，所用引物均位于对应基因的预测剪切位点下游约200~300核苷酸处。将所得PCR产物进行琼脂糖凝胶电泳检测。
提供机构：
figshare
创建时间：
2023-01-19
