Optical Control of Peroxisomal Trafficking

The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.燕麦向光素1（Avena sativa phototropin1）的蓝光响应LOV2结构域（AsLOV2）此前已被用于通过光信号调控多种蛋白靶标的活性与结合特性。本研究利用AsLOV2对过氧化物酶体靶向序列（peroxisomal targeting sequence）实施光笼蔽修饰，实现了对过氧化物酶体蛋白导入过程的光控调节。我们构建了一款蛋白标签LOV-PTS1，该标签可融合至目标蛋白上，使目标蛋白能够在光照条件下被定向导入过氧化物酶体。该方法为在用户指定的时间于特定细胞中诱导触发过氧化物酶体蛋白转运提供了可行途径。