The phosphorylation of FOXA1-S331 is indispensable for FOXA1-AR dependent cistrome [RNA-seq]

To define the function of FOXA1-S331 phosphorylation, we substituted S331 in both FOXA1 alleles for alanine in C4-2 cells via CRISPR/Cas9-based gene editing, thereby generating phosphorylation incompetent FOXA1S331A cells. We directly assessed the consequences of phospho-incompetent FOXA1S331A mutation on the transcription profile, chromatin deposition of FOXA1, AR, H3K27ac and chromatin accessibility. Overall design: Comparative gene expression profiling analysis of RNA-seq data for C4-2 cells and its mutiaon derivative (FOXA1S331A).

为阐明叉头框蛋白A1（FOXA1）S331位点磷酸化的功能，我们通过基于CRISPR/Cas9的基因编辑技术，将C4-2细胞中两个FOXA1等位基因的S331位点替换为丙氨酸，由此构建得到磷酸化缺陷型FOXA1S331A细胞系。
我们直接评估了磷酸化缺陷型FOXA1S331A突变对转录谱、FOXA1、雄激素受体（AR）、组蛋白H3赖氨酸27乙酰化（H3K27ac）的染色质定位以及染色质可及性的影响。
整体实验设计：对C4-2细胞及其突变衍生细胞系（FOXA1S331A）的RNA测序（RNA-seq）数据开展比较基因表达谱分析。