Reduced antigen-triggered BCR signaling in Fcmr-deficient B cells

Differential gene expression profile between WT and Fcmr KO splenic B cells after BCR stimulation Purified B cells from WT and Fcmr KO mice were cultured with 10 μg/mL F(ab’)2 anti-IgM and harvested at 1 or 6 h for RNA-seq

本数据集为B细胞受体（B Cell Receptor, BCR）刺激后，野生型（Wild Type, WT）与Fcmr基因敲除（Knock Out, KO）小鼠脾脏B细胞间的差异基因表达谱。实验方法为：从野生型及Fcmr基因敲除小鼠体内分离纯化脾脏B细胞，以10 μg/mL的F(ab’)2片段抗免疫球蛋白M（IgM）进行培养，分别于刺激后1小时和6小时收集样本，用于RNA测序（RNA-seq）。