Expression data from P2 mouse hippocampus

Zinc finger protein ZBTB20 plays a critical role in mouse hippocampal development by orchestrating gene expression profile of hippocampal neurons. We used microarrays to investigate the target gene of ZBTB20 in mouse hippocampal development. Mouse hippocampi were harvested at postnatal day 2 for RNA extraction and hybridization on Affymetrix microarrays. We sought to identify the target genes of transcription factor ZBTB20 in hippocampal development. To that end, we isolated the hippocampi from ZBTB20 knockout and their littermate control mice.锌指蛋白ZBTB20（Zinc finger protein ZBTB20）可通过调控海马神经元的基因表达谱，在小鼠海马发育过程中发挥关键作用。我们采用基因芯片技术，探究锌指蛋白ZBTB20在小鼠海马发育中的靶基因。实验于出生后第2天采集小鼠海马组织，用于RNA提取并在Affymetrix基因芯片上进行杂交反应。本研究旨在鉴定转录因子ZBTB20在海马发育过程中的靶基因，为此我们分别从ZBTB20基因敲除小鼠及其同窝对照小鼠中分离海马组织。