Trans-generational effect of protein restricted diet on adult body and wing size of Drosophila melanogaster

Protein restriction (PR) has established feasible trade-offs in Drosophila melanogaster to understand lifespan or aging in a nutritionally challenged environment. However, the phenotypes of body size, weight and wing length respond according to factors such as flies’ genotype, environmental exposure, and parental diet and hence their understanding is essential. Here, we demonstrate the effect of long-term PR diet on body size, weight, normal & dry wing length of flies subjected to PR50 and PR70 (50% and 70% protein content present in control food respectively) for 20 generations from pre-adult stage. We found that PR fed flies have lower body weight, relative water content (in males), unaltered (PR50%) and higher (PR70%) relative fat content in males, smaller normal and dry body size as compared to control and generations 1 and 2. Interestingly, wing size and pupal size of PR flies are smaller and showed significant effects of diet and generation. Thus, these traits are sex and gene...

蛋白质限制（Protein restriction, PR）已在黑腹果蝇（Drosophila melanogaster）中建立了可行的权衡模型，用于探究营养胁迫环境下的寿命或衰老机制。然而，果蝇的体型、体重与翅长等表型会随果蝇基因型、环境暴露情况以及亲代饮食等因素发生响应，因此对这些表型的解析至关重要。本研究针对从成虫前期开始连续20代暴露于PR50和PR70（分别为对照饲料中蛋白质含量的50%和70%）的果蝇，探究了长期蛋白质限制饲料对其体型、体重、正常翅长与干翅长的影响。研究发现，与对照组以及第1、2代相比，蛋白质限制饲喂的果蝇体重更低，雄性个体的相对含水量下降；雄性个体的相对脂肪含量在PR50组无显著变化，在PR70组则显著升高，且其正常体型与干重体型均更小。值得注意的是，蛋白质限制组果蝇的翅尺寸与蛹尺寸均更小，且表现出饮食处理与世代数的显著影响。综上，这些性状受到性别与基因的……