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Yeast spotting assay with ERG7 deficient gil77 or gil77::CAS1 strains.

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Figshare2016-02-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Yeast_spotting_assay_with_ERG7_deficient_gil77_or_gil77_CAS1_strains_/1217685
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资源简介:
Growth of gil77 yeast strain transformed with the pYeDP60 vector or pYeDP60-NtCAS1 was tested on SGal medium supplemented or not with 20 µg/ml of ergosterol. After 5 days at 30°C, plates supplemented with ergosterol showed fully-grown colonies, whereas inductive conditions (SGal without ergosterol) allowed only limited growth of yeast expressing NtCAS, indicating however that NtCAS1 expression partially overcomes ERG7 deficiency.
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2016-02-23
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